High Throughput Process Optimization
High throughput process development has become an essential element in the design and development of modern downstream processes. This technique shortens development time and increases the process knowledge available during the early phase of a development program.
Historically, the identification of a specific ligand for a target protein has required the engagement of specialist companies. Whilst this approach can be very successful, such studies often require some time to set-up and perform as well as requiring specific details of the target protein and sample materials to be evaluated.
More recently, a limited number of very general ligands, sometimes described as mixed-mode ligands, have been developed incorporating simple ion-exchange (IEX) and hydrophobic (HIC) functionalities into a single ligand structure. These types of ligand have been particularly successful for general polishing/host cell protein (HCP) removal applications and have the advantage that users can undertake the valuations themselves in-house, using standard laboratory equipment, in a relatively short time frame.
ProMetic BioSciences Ltd (PBL) have combined the advantages of both of these approaches resulting in the Multi-Mode Mimetic Ligand™ Library – an easy to use, rapid screening toolbox that can be used to identify chromatography adsorbents for a wide variety of applications, including both product capture and polishing.
The multi-mode screening library consists of a 96-micro column block (PuraPlate™) containing a diverse array of stable synthetic ligands, arranged into 6 different zones (shown above), which can be screened in chromatographic (flow-through) mode against a wide range of starting feed stocks.