Protein Technologies – Bioseparation Products
FAbsorbent™ - A new synthetic ligand affinity adsorbent for antibody fragment purification
- High-capacity
- Protein L alternative
- Robust affinity adsorbents, easily sanitizable with Sodium Hydroxide
- For purification and/or antibody fragments capture
- High linear flow rates with PuraBead® cross-linked agarose matrix
Figure 1: Fabsorbent™ F1P ligand interaction with antibody light chain
In response to the growth of antibody fragmentsas research tools and bio therapeutic products, ProMetic has developed a new affinity adsorbent for the capture and purification of a wide range of antibody fragments. FAbsorbent™ F1P HF is a synthetic-ligand light-chain binding adsorbent which can be used as a superior alternative to Protein L for the capture and purification of antibody fragments including monovalent antibody fragments (e.g. Fab, scFv), engineered antibody variants and single domain antibodies.
The robustness and broad applicability of FAbsorbent™ F1P HF makes this adsorbent the ideal choice for both early stage research and development applications and also for larger-scale manufacturing uses where the full benefit of the high-flow/low pressure Purabead® HF support matrix can be utilised. Given its broad selectivity, FAbsorbent™ F1P HF provides a “platform” technology for the purification of antibody fragments.
Specifications
| Ligand: | F1P Synthetic triazine ligand |
| Matrix: | PuraBead® P6HF (6% near-monodisperse highly cross-linked agarose) |
| Particle size: | 90 ± 10µm |
| Binding capacity: | Up to 60mg human IgG/ml and 20 mg Fab/ml resin (dependant on antibody/fragment type and source) |
| Operational Flow rate: | Up to 500cm/hr |
| Operating Pressure: | Up to 3 bar (45psi) |
| Operating pH: | pH 1.5 - 14 |
| pH Stability: | Long term (3 months): pH 3 - 13 |
| Chemical stability: | All commonly used aqueous buffers and co-solvents |
| Sanitization: | 0.5 - 1M sodium hydroxide, 25°C |
| Storage: | 20% ethanol |
Ordering Information
FAbsorbent™ F1P HF slurry for column packing
| FAbsorbent™ F1P HF (25 mL) | 3904-00025 |
| FAbsorbent™ F1P HF (100 mL) | 3904-00100 |
| FAbsorbent™ F1P HF (500 mL) | 3904-00500 |
| FAbsorbent™ F1P HF (1000 mL) | 3904-01000 |
FAbsorbent™ F1P HF pre-packed columns (for attachment to chrmatography workstations)
| F1P HF Screening Columns (5 x 1mL) | tba |
FAbsorbent™ F1P HF PuraPlate™ (96 column plate for rapid antibody screening and purification method development)
| F1P HF PuraPlate™ (96 column plate) | tba |
ProMetic offers a range of larger pack sizes for supply of bulk resins into development and regulated cGMP manufacturing processes. For more information on FAbsorbent™ F1P HF and supply related matters please do not hesitate to contact us.