Protein Technologies – Bioseparation Products
MAbsorbents® - Synthetic affinity ligand adsorbents for antibody purification
- High-capacity
- Protein A alternative
- Total absence of animal and cell-derived contamination
- Robust affinity adsorbents, easily sanitizable with NaOH
- For purification and/or primary antibody capture
- Optimum antibody selectivity from a range of sources
- High linear flow rates with PuraBead® cross-linked agarose matrix
It's no secret that antibodies dominate the stage when it comes to biotech products under development, in clinical trials and in therapeutic use. This demand for antibodies places enormous pressure on purification process designers to remove contaminants, improve product yields, increase purity, reduce costs and meet stringent regulatory requirements.
Traditionally researchers and manufacturers have turned to Protein A as the principal antibody purification technology. However, increasing concerns about the limitations of Protein A, including leaching into the product, antibody denaturation, poor stability under harsh process conditions, the nature of its biological source, and its high cost, have resulted in the need for a suitable alternative.
Our MAbsorbent® synthetic affinity ligand adsorbents are the innovative alternative to Protein A. While maintaining the requirements for binding capacity, product yield and purity, MAbsorbents® can operate in harsh environments for hundreds of process cycles at less than half the cost of traditional purification methods.
MAbsorbent® A1P and A2P – your high performance alternatives to Protein A
MAbsorbent® synthetic affinity ligand adsorbents are validated for the purification of antibodies. Designed to improve efficiency over traditional Protein A processes, these totally synthetic Mimetic LigandsTM are derived from our Chemical Combinatorial LibraryTM of multi-dimensional triazine derivatives.
Similar but very different
Its true. MAbsorbent® synthetic affinity ligand adsorbents mimic recombinant and natural Protein A. However, they are very different in that MAbsorbents bind to all sub-classes of IgG, including IgG3. MAbsorbents also have different species specificity compared to Protein A.
Explore the difference
MAbsorbents® were developed to mimic the Phe-132, Tyr-133 dipeptide binding site in the hydrophobic core structure of Protein A. We have developed IgG binding ligand libraries using a triazine scaffold substituted with aromatic amines. The resultant synthetic bifunctional ligands exhibit high affinities for human immunoglobulin G. Hence, MAbsorbent® A1P and A2P provide excellent purification performance. The more you explore the unique differences to Protein A, the more you will realize MAbsorbents® are the logical first choice for the isolation of antibodies.
Protein A Binding Site
MAbsorbent A1P Ligand
MAbsorbent A2P Ligand
Discover more advantages
MAbsorbent® synthetic affinity ligands offer many advantages over protein and peptide ligands. For example, there are no origin traceability issues. Due to the absence of animal and cell-derived contaminants as well as host cell proteins, DNA and cell culture or fermentation components, the threat of potential contamination is minimized. This of course, reduces process validation costs.
Another key difference is the robust nature of MAbsorbents® which are easily sanitizable with up to 1M NaOH. MAbsorbents® are also compatible with other common elution solvents such as ethylene glycol. All of these advantages, yet MAbsorbents® are much more economical than protein-based affinity chromatography media.
Similar binding capacity, more flexibility
MAbsorbents® effectively bind a wide variety of human and mammalian polyclonal antibodies (including bovine, mouse, sheep, goat, horse and rabbit) as well as whole monoclonal antibodies, humanized antibody chimeras and antibody fragments. MAbsorbent® A2P in particular is normally recommended for human or humanized antibodies, while MAbsorbent® A1P has proven ideal for murine antibodies.
Static binding capacity of MAbsorbents® is comparable to Protein A typically up to 50mg pure human IgG/mL settled gel. Static and dynamic binding capacities vary, of course, with the type of antibody and the nature of the feed stream. This is where MAbsorbent® flexibility takes over. A simple pre-conditioning step in the MAb feed improves binding when dealing with challenging cell culture additives such as Phenol Red or Pluronic. Very dilute feeds can be concentrated to improve binding.
Go with the flow
MAbsorbent® synthetic affinity adsorbents enable high flow rates due to the tightly controlled particle sizing of our PuraBead®, cross-linked, agarose matrix. Our proprietary cross-linking and ligand derivatization process maximizes linear flow while maintaining binding capacity levels.
Different antibody process streams, different sources, one solution...
MAbsorbent® A1P and A2P can be used to capture IgG from a wide variety of process streams including serum, plasma, ascites fluid, mammalian cell culture supernatant, or transgenic sources. MAbsorbents® may be used for direct capture of antibodies, or as part of a multistep process depending on the specific nature of the feedstream and process requirements. For purification of MAbs, the binding pH range for MAbsorbents® is between pH 6 to 8, relatively independent of salt concentration. Elution normally occurs at a pH range of 2 to 4, but alternative elution strategies may be employed for near-neutral operations.
Specifications
| Ligand: | Synthetic, aromatic triazine derivative |
| Matrix: | PuraBead 6XL: 6% cross linked agarose |
| Particle size: | 100 ± 25µm (90%) |
| Binding capacity: | Up to 50mg human IgG/ml resin, 20-30mg MAb/ml resin |
| Flow rate: | Up to 600cm/hr |
| Operating Pressure: | 1 bar (15psi) |
| Operating pH: | pH 1.5 - 14 |
| pH Stability: | Long term (3 months): pH 3 - 13 |
| Chemical stability: | All commonly used aqueous buffers |
| Sanitization: | 1M NaOH, 25°C |
| Sterilizability: | Autoclavable: 121°C, 20 minutes |
| Storage: | 20% ethanol |
Ordering Information
MAbsorbent® A1P/A2P Suspension for packing into columns
| A1P (25 ml) | 3900-00025 |
| A1P (100 ml) | 3900-00100 |
| A1P (500 ml) | 3900-00500 |
| A1P (1000 ml) | 3900-01000 |
| A2P (25 ml) | 3901-00025 |
| A2P (100 ml) | 3901-00100 |
| A2P (500 ml) | 3901-00500 |
| A2P (1000 ml) | 3901-01000 |
MAbsorbent® A1P/A2P Pre-packed columns for attachment to chromatography workstations
| A1P Screening Columns (5 x 1 ml) | 4900-00001 |
| A1P Screening Columns (1 x 10 ml) | 4900-00010 |
| A2P Screening Columns (5 x 1 ml) | 4901-00001 |
| A2P Screening Columns (1 x 10 ml) | 4901-00010 |
MAbsorbents® are supplied in 20% ethanol as preservative
For more information, please contact us.