Protein Technologies – Bioseparation Products
Other Affinity Adsorbents
AMINOPHENYL BORONATE AGAROSE 6XL
Aminophenyl Boronate agarose 6XL has been developed as a versatile tool for many biochemical studies. Advantages of this affinity adsorbent from ProMetic include:
GOOD CHEMICAL STABILITY - INEXPENSIVE
- LARGE BATCH AVAILABILITY
- HIGH CAPACITY
- AUTOCLAVABLE
- MANUFACTURED TO GMP / ISO 9001:2000 QUALITY STANDARDS
Ionized boronic acids have a unique affinity to form reversible complexes with 1,2-diols, 1,3-diols, 1,2-hydroxy acids and 1,2- hydroxylamines. These functionalities are often found in biomolecules and therefore, boronate affinity chromatography is a powerful step in the purification of diverse molecules including carbohydrates, nucleic acids (nucleosides, nucleotides and RNA's), polyphenols (catechols, flavonoids), certain enzymes (serine proteinases) and other proteins. The affinity ligand is bonded onto 6% cross-linked agarose by a highly stable bonding method to provide a robust adsorbent capable of withstanding NaOH cleaning procedures.
Specifications
| Support Matrix: | 6% cross-linked agarose |
| Bead Size: | 45 - 165 microns |
| Exclusion Limit: | 4 x 106 Daltons |
| Temperature Range: | 4 - 120°C |
| pH Range: | 3 - 11 (continuous) |
| 2 - 14 (intermittent) | |
| Maximum Pressure: | 15 psi (1 bar) |
| Storage: | 4°C in solution of ethanol/0.1M NaCl (25:75v/v) |
| Cleaning/Depyrogenation: | 20mM acetic acid/1M NaOH |
| Chemical Compatibility: | Compatible with most buffers and common additives. |
| Sterilization: | 1M NaOH (2 hours); autoclave 30 min., 120°C, pH 7.0 |
For more information, please contact us.
AMINOBENZAMIDINE AGAROSE 6XL
Aminobenzamidine Agarose 6XL is the perfect tool for your protease and esterase purification requirements. Advantages of this affinity adsorbent include:
- SPECIFIC FOR TRYPSIN-LIKE SERINE PROTEASES
- HIGH CAPACITY
- GOOD CHEMICAL STABILITY
- INEXPENSIVE
- AUTOCLAVABLE
- LARGE BATCH AVAILABILITY
- MANUFACTURED TO GMP / ISO 9001:2000 QUALITY STANDARDS
Aminobenzamidine is a potent synthetic inhibitor of trypsin-like serine proteases. Immobilized aminobenzamidine has been used in the purification of a large number of serine proteases and esterases by affinity chromatography including trypsin, thrombin, kallikrein, urokinase, acetyl cholinesterase and alkaline phosphatase. Aminobenzamidine Agarose 6XL may also be used in the separation of proteases (for example, trypsin and chymotrypsin) and in the removal of trace protease contamination from your valuable final product. This agarose-based affinity adsorbent offers the superior performance and stability characteristic of all ProMetic's adsorbents.
Properties
Stability
Aminobenzamidine is immobilized to 6% cross-linked agarose via a spacer epoxy linkage that is highly stable over a wide pH range. The aminobenzamidine group degrades at pH's higher than 12, a process accelerated by light. Therefore avoid prolonged exposure to strong alkali solutions and ideally store the adsorbent in the dark below pH 9.
Ligand Content
7.5 +/- 1.0µMol aminobenzamidine per 1ml moist gel.
Capacity
Aminobenzamidine Agarose 6XL binds 25-40mg of trypsin per ml gel depending on the sample condition and pH.
Flow Properties
Aminobenzamidine Agarose 6XL offers the superior mechanical rigidity of all ProMetic's agarose-based adsorbents.
| Material | Max. Recommended Pressure | Max. Linear Flow Rate* |
|---|---|---|
| 6% Agarose | 0.5 bar | 100 cm/hr |
| 6% Cross-linked Agarose | 0.7 bar | 180 cm/hr |
| PABA Agarose (ProMetic) | 1.2 bar | 300 cm/hr |
* Linear flow rates for a 14-15 cm bed height axial column
Regeneration
Aminobenzamidine Agarose 6XL can be sanitized before column packing with 70% (v/v) ethanol and can be fully regenerated with 8M urea.
Storage
Aminobenzamidine Agarose 6XL is supplied in 0.1M sodium chloride/ethanol [75/25 (v/v)] and should be stored at 4°C until required. After use, re-equilibrate with preservative and store in the dark at 4°C.
Ordering Information
p-Aminobenzamidine Agarose 6XL
| Volume | Part No. |
|---|---|
| 25ml | 0320-00025 |
| 50ml | 0320-00050 |
| 100ml | 0320-00100 |
| 250ml | 0320-00250 |
| 500ml | 0320-00500 |
For more information, please contact us.
AVIDIN AGAROSE 4XL
Avidin is a basic (pI=10) glycoprotein comprised of four identical subunits having a combined molecular weight of about 67,000 (sub unit molecular weight 15,600). Its interaction with biotin is the strongest known biological interaction between protein and ligand (Ka=1015M-1). The formation of this interaction is rapid, and once formed, it is highly stable and cannot be disrupted by extremes of temperature, pH, organic solvents or denaturing agents. This unique property has provided a valuable tool for development of immunoassays, receptor studies, nucleic acid immobilization, affinity cytochemistry and protein isolation.
For chromatographic applications, the strong interaction of biotin-avidin is a major drawback. Biotinylated proteins are recovered from avidin columns only under denaturing conditions. A number of strategies to overcome this limitation exist. ProMetic has built up a strong expertise in development and manufacture (to GMP/ISO 9001:2000 Quality Standards) of stable agarose adsorbents, which are used in affinity chromatography and protein immobilizations.
For more information, please contact us.