Protein Technologies – Bioseparation Products
Purification and Screening Kits
- PuraPlate™ Ligand Screening Kit
- PIKSI M System Kit (Mimetic Ligand screening kit)
- PIKSI Screening Column Kits (For Chromatography work stations: screening, method scouting & development)
- Plasmid DNA Endotoxin Removal Ki
PuraPlate™ Ligand Screening Kit
| Multiple Media Plates | ||
|---|---|---|
| Mimetic Ligand Screening Kit | Synthetic Affinity Ligands including Mimetics Red® 2,3, Orange 1,2,3, Yellow 1,2, Green 1, Blue 1. | 211 |
| Single Media Plates | Mimetics | |
| Mimetic Red® 2 (0020) | Synthetic Affinity Ligand | 2209 |
| Mimetic Red® 3 (0013) | Synthetic Affinity Ligand | 2211 |
| Mimetic Orange® 1 (0030) | Synthetic Affinity Ligand | 2212 |
| Mimetic Orange® 2 (0040) | Synthetic Affinity Ligand | 2213 |
| Mimetic Orange® 3 (0050) | Synthetic Affinity Ligand | 2214 |
| Mimetic Yellow® 1 (0060) | Synthetic Affinity Ligand | 2215 |
| Mimetic Yellow® 2 (0070) | Synthetic Affinity Ligand | 2216 |
| Mimetic Green® 1 (0080) | Synthetic Affinity Ligand | 2217 |
| Mimetic Blue® 1 (0090) | Synthetic Affinity Ligand | 2218 |
| Mimetic Blue® AP A6XL (0110) | Synthetic Affinity Ligand | 2221 |
| Mimetic Blue® SA A6XL (0120) | Synthetic Affinity Ligand | 2219 |
| Mimetic Blue® SA A6XL (3120) | Synthetic Affinity Ligand | 2220 |
| Single Media Plates | IEX | |
| DEAE PuraBead® HF (3451) | Weak Anion Exchanger | 2202 |
| SP PuraBead® HF (3453) | Strong Cation Exchanger | 2208 |
| Single Media Plates | HIC | |
| Phenyl Agarose 6XL (0405) | 6-carbon ring hydrophobic | 2222 |
| Butyl Agarose 6XL (0415) | 4-carbon ring hydrophobic | 2223 |
| Hexyl Agarose 6XL (0425) | 6-carbon ring hydrophobic | 2224 |
| Octyl Agarose 6XL (0435) | 8-carbon ring hydrophobic | 2225 |
| Decyl Agarose 6XL (0445) | 10-carbon ring hydrophobic | 2226 |
| Single Media Plates | MAbsorbents® | |
| MAbsorbent® A1P (3900) | Antibody purification, preferably murine | 2203 |
| MAbsorbent® A2P (3901) | Antibody purification, preferably human/humanized | 2204 |
| MAbsorbent® A2P HF (3903) | Antibody purification, preferably human/humanized | 2205 |
| Single Media Plates | Affinity | |
| Para-aminobenzamidine 6XL (0320) | Purification or removal of serine proteases | 2206 |
| Aminophenylboronate 6XL (0355) | Purification or removal of glycoproteins or other entities containine 1,2 and 1,3 cis-diol moieties | 2207 |
PIKSI™ M System Kit
- INTEGRATED SCREENING KIT CONTAINS 10 DIFFERENT AFFINITY MEDIA
- FAST AND SIMPLE SELECTION -- EASY SCALE-UP
- DMFs, GMP ISO 9001:2000 QUALITY
- PROPRIETARY BONDING CHEMISTRY VIRTUALLY ELIMINATES LEAKAGE
- WIDE RANGE OF SELECTIVITY WITH COMPUTER MODELED LlGANDS FOR AFFINITY PURIFICATION OF DIVERSE PROTEINS
- REUSABLE MODULE AND MEDIA CAN BE REGENERATED WITH 1M NAOH
- MIMETIC™ MEDIA AVAILABLE INDIVIDUALLY IN LARGER PACKS AND IN BULK FOR PROCESS APPLICATIONS
The broadly specific Mimetic™ affinity adsorbents show different binding properties toward any given target protein molecule. These specificity differences are exploited in the PIKSI™ (Protein Isolation Kit for Sorbent Identification) Kit, which has been developed to circumvent the most arduous task of a research project, namely the development of an optimized chromatographic procedure for protein purification, without any prior knowledge of the molecules physio-chemical properties (e.g. iso-electric point, molecular weight, hydrophobicity, ligand specificity).
Designed MIMETIC™ Affinity Ligands - Good Selectivity, Wide Application Range
Affinity chromatography provides a high degree of purification in one step. Although widely used for a number of separations, textile-dye based ligands have not been suitable for large scale purifications. This new generation of computer-modeled synthetic MIMETIC™ ligands offers a range of rationally designed biomimetic structures that interact more specifically with proteins and enzymes. Mimetic™ media easily replace dye-based gels for purification of enzymes and blood proteins, especially those that interact strongly with nucleotides and co-factors.
Improved Stability, Flow Rates and Economy
Mimetic™ media can be reused for many cycles because they can tolerate routine sterilization and regeneration with 1M NaOH. An extremely stable, proprietary bonding chemistry virtually eliminates the ligand leakage problems found with dye-based sorbents. You can also use MIMETIC™ adsorbents at flow rates up to 3 times that of conventional 6% cross-linked agaroses, resulting in increased throughput. Synthetic MIMETIC™ ligands are highly defined and consistent, so you get stability and reproducibility at an economic price. Now you can reduce the cost of your purification with an effective affinity step.
High Capacity and Better Recovery of Activity
The design of the Mimetic™ ligands means higher capacity (up to 50mg protein/ml gel). Mimetic™ ligands minimize loss of activity due to conformational changes, and adsorption and desorption can be carried out under physiological conditions.
Simplify Method Development for Easy Scale-Up
The ultimate goal when purifying proteins is either to provide answers to fundamental questions pertaining to molecular structure and function or to provide bulk commodity products for use in other application (e.g. therapeutics, biotransformations). The development of an optimized chromatographic separation protocol is often the most challenging aspect of such a project due to the molecular diversity of proteins and complexity of biological samples containing the target molecule.
The PIKSI™ M Kit makes fast work out of selecting the optimal MIMETIC™ ligand and conditions. The kit contains ten columns that can be operated simultaneously under gravity flow or individually with a peristaltic pump. Use the kit for method scouting, method development, and then simply scale-up. The adsorbents are manufactured under full GMP and ISO9001:2000 quality systems, and Drug Master Files (DMF) have been filed to make traceability and compliance easier for you. The MIMETIC™ media are available in pack sizes from 25ml to 500L single batches to ensure reliability in any application, from lab scale to large bioprocess.
The PIKSI™ M Kit is supplied with ten packed MIMETIC™ adsorbents and offers the following benefits:
- Rapid screening to identify adsorbents suitable for affinity purification of the target molecule either in the positive mode (binds the target molecule) or negative mode (binds the contaminants).
- The broad specificity of Mimetic™ adsorbents with diverse protein binding properties offers a high level of certainty of identifying a suitable adsorbent in a single experiment.
- Following adsorbent identification binding elution and conditions (pH, ionic strength) can be optimized using the PIKSI™ Kit.
Further method development and small-scale purification may be carried out on our pre-packed 10ml columns or with Mimetic™ adsorbent slurry packed into any conventional chromatography columns.
Typical Proteins Purified by Mimetic Ligand Adsorbents
| Human Serum Albumin | Proteases |
| Blood Proteins | Oxidases |
| Dehydrogenases | Ligases |
| Kinases | Antibodies |
| Nucleases | Alpha-1 antitrypsin |
Specifications
| Support Matrix: | 6% cross-linked agarose with fast-flow properties |
| Bead Size: | 45 - 165 microns |
| Exclusion Limit: | 4 x 106 Daltons (6XL) |
| Temperature Range: | 4-120° C |
| pH Range: | 3 - 13 (continuous) 2 - 14 (intermittent) |
| Storage: | 4° C in a solution of ethanol/0.1 M NaCl (25:75 v/v) |
| Cleaning/Depyrogenation: | 1M NaOH |
| Chemical Compatibility: | Compatible with alcohols, esters, ketones, ethers, and most biological buffers; not harmed by common buffer additives such as chaotropes, detergents, chelating agents, thiols. |
| Sterilization: | 1M NaOH; or autoclave, 30 min. at 120°C in a neutral buffer (50 mM sodium phosphate, pH 7.0). |
| Protein Binding Capacity: | Can exceed 50mg of protein per ml of packed gel. Varies with applied sample. |
| Maximum Pressure: | 10 psi (6XL) |
| Void Volume: | 0.4ml for individual columns; 2.0 ml for individual reservoirs. |
| Chemical Resistance of Kit Body: | Compatible with most buffers, buffer salts, chaotropic ions and alkalis; incompatible with mineral acids, concentrated organic acids, and organic solvents. |
| Dimensions: | 9" x 6" X 2" (w x h x d) (23cm x 15.25cm x 5cm) |
| Weight: | 1.1 lbs. (500 g) |
Ordering Information
Each kit contains an integral buffer reservoir, a detachable collection rack, and ten one-ml pre-packed columns of ten different Mimetic adsorbents in an integrated module.
| Item | Column Volume | Part No. |
|---|---|---|
| PIKSI™ -M SYSTEM | 1ml | 1000-00010 |
PIKSI™ Screening Column Kits
An all purpose range of pre-packed, re-useable screening columns, the PIKSI column concept is a fast and accurate solution to many purification problems.
Based on our high performance Agarose matrices, these columns offer:
- 1 ml volumes
- Packed with most of the ProMetic media currently available
- Convenient to use
- Fast, simple, affordable
- Run with all commercially available instruments, a simple peristaltic pump or just a syringe
- Easy to scale up
- Compatible around the World
Ready-to-use PIKSI columns are manufactured in biocompatible Delrin, and include porous tops and bottom frits, allowing high flow rates and avoiding dry out when the columns are run under gravity.
Ordering Information
| Product Description | Catalog Number/Quantity |
|---|---|
| Mimetic PIKSI M Screening Column Kit (The PIKSI M Screening kit supplied as an individual column set, 10 x 1ml Mimetics) | 1003-00001 |
| Hydrophobic PIKSI H Screening Column Kit (The PIKSI Screening kit supplied as an individual column set, 10 x 1ml HIC) | 1403-00001 |
5 x 1ml Column Screening Kits
| Product Description | Part No. | Product Description | Part No. |
|---|---|---|---|
| Mimetic Red 2 A6XL | 1020-00001 | Phenyl Agarose 6XL | 1405-00001 |
| Mimetic Red 3 A6XL | 1013-00001 | Butyl Agarose 6XL | 1415-00001 |
| Mimetic Orange 1 A6XL | 1030-00001 | Hexyl Agarose 6XL | 1425-00001 |
| Mimetic Orange 2 A6XL | 1040-00001 | Octyl Agarose 6XL | 1435-00001 |
| Mimetic Orange 3 A6XL | 1050-00001 | Decyl Agarose 6XL | 1445-00001 |
| Mimetic Yellow 1 A6XL | 1060-00001 | ||
| Mimetic Yellow 2 A6XL | 1070-00001 | ||
| Mimetic Green 1 A6XL | 1080-00001 | ||
| Mimetic Blue 1 A6XL | 1090-00001 | ||
| Mimetic Blue 2 A6XL | 1100-00001 | ||
| Mimetic AP A6XL | 1110-00001 | ||
| Mimetic Blue SA A6XL | 1120-00001 | ||
| pAB Agarose 6XL | 1320-00001 | ||
Plasmid DNA Endotoxin Removal Kit
Integrated purification kit that removes endotoxin while purifying plasmid DNA
- Endotoxin < 3 EU/mg
- Rapid user protocol - 7 minute preps
- pDNA capacity> 0.75mg per prep
- No surfactants
- Proven purification technology scaleable to larger preps
- Economical single-use syringe format
ProMetics Plasmid DNA Endotoxin Removal Kit is based on Perfluorosorb® S purification media, already proven in large-scale plasmid purification. Perfluorosorb is a highly engineered, polymeric adsorbent that purifies DNA through a reverse phase chromatographic mechanism, and can be applied to primary capture, cccDNA enrichment and endotoxin removal.
Endotoxin levels of < 3EU/mg can be achieved with a single pass rapid procedure of only 7 minutes per prep, from starting levels of > 3000 EU/mg. High DNA capacity of > 3.75 mg and absence of surfactants results in the highest purity preps that will drive your research further.
Kit Contents
- 5 x Purification columns containing Perfluorosorb® S
- 25 mls of DNA Mixing Buffer
- 100 mls of Buffer C1
- 100 mls of Buffer C2
- 100 mls of Buffer C3
- 10 Syringes
- Protocol Card
- User Manual
- Material Safety Data Sheet
Specifications
- Binding Capacity: > 3.75 mg DNA
- pH Stability: 1 - 14
- Storage conditions: Room temperature
Ordering Information
Plasmid DNA Endotoxin Removal Kit: Part No. 5101
For more information, please contact us.